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Miltenyi Biotec MACSima imaging platform

Miltenyi Biotec MACSima imaging platform

  • Fully automated high-content imaging based on fluorescence microscopy
  • Analysis of hundreds of markers on a single sample
    • Samples are stained with fluorochrome-conjugated antibodies
    • Epifluorescence images are acquired from the target area
    • The fluorescence signal is erased, and the process restarts automatically
  • Processing all types of fixed samples (tissue, single cells)
  • Automated processing and imaging
  • Software for analysis of high-dimensional data

 

For more information about this equipment and its setup please contact:

Martina Esser
Research Technician

Tel: +49 941 944–38117
Email: martina.esser@ukr.de

Leica STELLARIS 8

Leica STELLARIS 8

 

  • Multi-color confocal microscopy and live-cell imaging
  • Controlled humidity, temperature, CO2 and O2
  • High-resolution 3D reconstructions
  • Highest frame rates to capture rapid dynamic events
  • White Light Laser: excitation range 440-790nm up to NIR
  • Up to 8 single excitation wavelengths simultaneously
  • Detection range: 410-850nm
  • Long-term live-cell imaging across FR spectrum with reduced phototoxicity on cultured cells
  • Dynamic Signal Enhancement and Lightening for superb image quality at full speed and in super-resolution, even for low-expressing molecules
  • TauSense lifetime tool, measuring mean average arrival time of photons
  • TauContrast for functional information (metabolic status, pH, ion concentration)
  • TauGating for improved image quality separating auto-fluorescence or reflection light
  • TauSeparation to distinguish spectrally overlapping fluorophores

 

For more information about this equipment and its setup please contact:

Dr. Maria Xydia
Postdoctoral Scientist

Tel: +49 941 944–18104
Email: maria.xydia@ukr.de

Leica LMD7

Leica LMD7 Laser Microdissection

  • Upright fluorescence microscope using laser beams to cut tissues and live-cell cultures
  • Movement of the laser, not the sample, for high laser precision during cutting
  • Sample collection using gravity—no sample contamination
  • Isolation of entire areas of tissue
  • Highly precise isolation of single-cells and subcellular structures such as chromosomes
  • Laser adjustment according to the sample (brain, bone)
  • Up to 8 fluorochromes in parallel
  • Incubator with controlled humidity, temperature and CO2
  • Compatible with downstream sequencing applications and subculturing of isolated cells

 

For more information about this equipment and its setup please contact:

Dr. Maria Xydia
Postdoctoral Scientist

Tel: +49 941 944–18104
Email: maria.xydia@ukr.de

 

MMI CellManipulator

MMI CellManipulator & CellEctor

 

CellManipulator

  • Optical tweezers system, infrared laser, 8W power
  • Use of mechanical forces from a strongly focused laser beam
  • Contact-free manipulation of microscopic particles, single living cells, or subcellular organisms
  • Hold, move, rotate, join, separate or stretch up to 2×10 objects (beads, particles, cells) of 0.2 to 200 microns
  • Measurement of binding forces, cell-to-cell interactions, and intracellular activities

 

CellEctor

  • Single-cell picking of living cells from liquid cell cultures using Capillary Cell Sorting
  • Incubator with controlled humidity, temperature and CO2
  • Up to 4 fluorochromes in parallel
  • Compatible with downstream sequencing applications and subculturing of isolated cells

 

For more information about this equipment and its setup please contact:

Dr. Maria Xydia
Postdoctoral Scientist

Tel: +49 941 944–18104
Email: maria.xydia@ukr.de